Andy Hoofnagle MD, PhD
Title:
Assistant Professor, Department of Laboratory Medicine
Email Address:
Background:
Andy Hoofnagle received his undergraduate education at Cornell University. He obtained his medical degree from the University of Colorado Health Sciences and his PhD from the University of Colorado, Boulder as part of the Medical Scientist Training Program. His PhD advisor was Natalie Ahn. During his residency and chief residency in Clinical Pathology in the Department of Laboratory Medicine at the University of Washington, he did his post-doctoral work in the laboratory of Jay Heinecke, Department of Medicine. Upon completion of his residency, he was promoted to Assistant Professor of Laboratory Medicine and now serves as the Director of Clinical Mass Spectrometry.
Focus:
Dr. Hoofnagle's laboratory focuses on using proteomic and metabolomic approaches to investigate the intersection of inflammation and lipid metabolism. His laboratory has built robust assays for the quantification of proteins in lipoproteins using targeted proteomic approaches to investigate the changes in the HDL proteome due to diabetes and obesity. They are pioneering the use of immunoaffinity peptide enrichment strategies of analyte-specific peptides from tryptic digests in the clinical analysis of low-abundance serum proteins. The laboratory has developed assays for small molecule analytes, including arginine, arginine metabolites, and vitamin D, which are being used in basic science and large-scale clinical studies. Several mouse models are being used to assess the importance of complement regulatory proteins in insulin sensitivity and lipid metabolism. All of these studies are in collaboration with outstanding investigators from the University of Washington and elsewhere.
Representative Publications:
Hoofnagle AN, Becker JO, Wener MH, and Heinecke JW. (2008) Quantification of Thyroglobulin, a Low-Abundance Serum Protein, by Immunoaffinity Peptide Enrichment and Tandem Mass Spectrometry. Clin Chem. 54:1796-804.
Marney LC, Laha TJ, Baird, GS, Rainey PM, and Hoofnagle AN. (2008) Isopropanol
Protein Precipitation for the Analysis of Plasma Free Metanephrines by Liquid Chromatography-Tandem Mass Spectrometry. Clin Chem. 54:1729-32.
Hoofnagle AN, Peterson GN, Kelly JL, Sayre CA, Chou D, and Hirsch IB. (2008) Utilization of Serum and Plasma Glucose Measurements as a Benchmark for Improved Hospital-wide Glycemic Control. Endocr Pract. 14:556-63.
Hoofnagle AN and Heinecke JW. (2009) Lipoproteomics: Using mass spectrometry-based proteomics to explore the assembly, structure, and function of lipoproteins. J Lipid Res. 50:1967-75.
Hoofnagle AN and Wener MH. (2009) The Fundamental Flaws of Immunoassays and
Potential Solutions Using Tandem Mass Spectrometry. J Immunol Methods. 347:3-11.
Current Collaborations:
Within the Diabetes and Obesity Center of Excellence and its Affiliated Members
Jay Heinecke
Tomas Vaisar
Alan Chait
Renee LeBoeuf
Outside the Diabetes & Obesity Center of Excellence
Robert Knopp, University of Washington
John Brunzell, University of Washington
Steven Kahn, Seattle VA Puget Sound Health Care System
Ian de Boer, University of Washington
Bryan Kestenbaum, University of Washington
Phyllis Wise, University of Washington
Larry Kessler, University of Washington
Tim Lyons, University of Oklahoma Health Sciences Center
Joshua Bornhorst, University of Arkansas
Leigh Anderson, Plasma Proteome Institute, Washington, DC
Terry Pearson, University of Victoria, BC
Lab Members:
Sean Agger, PhD
Jessica Becker, MT (ASCP)
Thomas Laha, MT (ASCP)